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. Author manuscript; available in PMC: 2020 Feb 5.
Published in final edited form as: Chem Res Toxicol. 2014 Apr 21;27(5):919–930. doi: 10.1021/tx500072m

Figure 2. Extension of a 32P-labeled 24-mer primer across G or N2-AnthG at position 25 by human wild-type and variant pol κ1−526 enzymes.

Figure 2.

Primer (24-mer) was annealed with each of the two different 36-mer templates, containing an unmodified G or N2-AnthG placed at the 25th position from the 3′-end. Reactions were done for 15 min with increasing concentrations of pol κ1−526 (0 − 1 nM) and DNA substrate (100 nM primer/template) as indicated. 32P-labeled 24-mer primer was extended in the presence of all four dNTPs (50 μM each). The reaction products were analyzed by denaturing gel electrophoresis and phosphorimaging. (A) Extension across G. (B) Extension across N2-AnthG.