Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Homo sapiens) | BUD23 | Gene ID: 114049 | Aliases: WBSCR22, MERM1 |
|
| Genetic reagent (Mus musculus) | Bud23null | This paper | Bud23null | Mutant allele: deleted exon 7 of BUD23 gene |
| Genetic reagent (Mus musculus) | BUD23fl/fl | This paper | BUD23fl/fl | Mutant allele: floxed exon 7 of BUD23 gene |
| Genetic reagent (Mus musculus) | Mck-Cre | Whitnall et al., 2008 | B6.FVB(129S4)-Tg(Ckmm-cre)5Khn/J | Heart and muscle specific Cre-driver |
| Cell line (Homo-sapiens) | A549 | ATCC | CCL-185 | Airway epithelial adenocarcinoma |
| Antibody | anti-BUD23 (Rabbit polyclonal) | GeneTex | Cat# GTX105840 | WB (1:500) |
| Sequence-based reagent | BUD23_F | This paper | Genotyping primers | 5’-CCTGGCTGATGTGTTGCTTT-3’ Annealing temperature: 62°C |
| Sequenced-based reagent | BUD23_R | This paper | Genotyping primers | 5’-GCTGCACATCTCTCCTCACT-3’ Annealing temperature: 62°C |
| Sequence-based reagent | MT-ND1_F | This paper | qPCR primers | 5’-GAAGTCACCCTAGCCATCATTC-3’ |
| Sequenced-based reagent | MT-ND1_R | This paper | qPCR primers | 5’GCAGGAGTAATCAGAGGTGTTC-3’ |
| Sequenced-based reagent | siRNA: nontargeting control 1 | Thermo Fisher | AM4611 | Silencer Select |
| Sequenced-based reagent | siRNA: nontargeting control 2 | Thermo Fisher | 4390847 |
Silencer Select |
| Sequenced-based reagent | siRNA: BUD23 |
Thermo Fisher | 41529 | Silencer Select |
| Sequenced-based reagent | siRNA: BUD23 | Thermo Fisher | 41530 | Silencer Select |
| Sequenced-based reagent | siRNA: BUD23 | Thermo Fisher | 41531 | Silencer Select |
| Software, algorithm | R | www.r-project.org | Key packages: -EDGER -BIOCONDUCTOR -EDASEQ |
|
| Commercial assay or kit | Seahorse XF Mito stress test | Agilent | 103015–100 | |
| Commercial assay or kit | Seahorse XF Glyco stress test | Agilent | 103020–100 |