Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Antibody | Anti-HA (Rabbit polyclonal) | Abcam | Cat#: Ab9110; RRID:AB_307019 | (1:133) |
| Antibody | Anti-IgG1(Mouse monoclonal) | Sigma | Cat#: M5284; RRID:AB_1163685 | (1:133) |
| Antibody | Anti-RPL22 (Rabbit polyclonal) | Invitrogen | Cat#: PA5-68320; RRID:AB_2692054 | (1:133) |
| Antibody | Anti-CD31(Rat monoclonal) | BD Pharmingen | Cat#: 550274; RRID:AB_393571 | (1:25) |
| Antibody | Anti-RAGE (Rabbit polyclonal) | Abcam | Cat#: Ab3611; RRID:AB_303947 | (1:3200) |
| Antibody | Anti-PTN (Mouse monoclonal) | Santa Cruz Biotechnology | Cat#: sc-74443; RRID:AB_1128556 | (1:3200) |
| Antibody | Anti-AQP7 (Rabbit polyclonal) | Novus Biologicals | Cat#: NBP1-30862; RRID:AB_2258607 | (1:3200) |
| Antibody | Anti-rat (Donkey polyclonal) | Invitrogen | Cat#: A-21208; RRID:AB_141709 | (1:300) |
| Antibody | Anti-rabbit (Donkey polyclonal) | Invitrogen | Cat#: A-21207; RRID:AB_141637 | (1:300) |
| Antibody | Anti-mouse (Goat polyclonal) | Invitrogen | Cat#: A11032; RRID:AB_2534091 | (1:300) |
| Chemical compound, drug | Lipopolysaccharide (LPS) | Sigma-Aldrich | Cat#: L2630 | |
| Chemical compound, drug | collagenase A | Roche | Cat#:10103586001 | |
| Chemical compound, drug | red blood cell lysis buffer | Biolegend | Cat#: 420301 | |
| Chemical compound, drug | Dynabeads | Invitrogen | Cat#: 11035 | |
| Chemical compound, drug | Collagenase/Dispase | Roche | Cat#: 11097113001 | |
| Chemical compound, drug | DNAse | Worthington Biochemical | Cat#: LK003170 | |
| Genetic reagent (M. musculus) | Mouse: Cdh5CreERT2/+; Rpl22HA/+ | This paper | Ref: Materials and methods – Experimental Animals | |
| Genetic reagent (M. musculus) | Rpl22HA/+ | Jackson Labs | JAX: 011029; RRID:IMSR_JAX:011029 | |
| Genetic reagent (M. musculus) | Cdh5CreERT2/+ | Jeong et al., 2017, Sörensen et al., 2009 | ||
| Other | Myelin Removal Beads | Miltenyl Biotec | Cat#: 130-096-433 | |
| Other | LS columns | Miltenyl Biotec | Cat#: 130-042-401 | |
| Other | CD31 microbeads | Miltenyl Biotec | Cat#: 130-097-418; RRID:AB_2814657 | |
| Other | MACS BSA Stock Solution | Miltenyl Biotec | Cat#: 130-091-376 | |
| Other | autoMACS Rinsing Solution | Miltenyl Biotec | Cat#: 130-091-222 | |
| Other | MS columns | Miltenyl Biotec | Cat#: 130-042-201 | |
| Other | ProLong Gold mounting medium | Invitrogen | CA#: P36934 | |
| Software, algorithm | Zen software | ZEISS | ||
| Software, algorithm | STAR v. 2.4.2 | Dobin et al., 2013 | ||
| Software, algorithm | HTSeq-count v. 0.6.1 | Anders et al., 2015 | ||
| Software, algorithm | biomaRt package v. 2.26.1 | Durinck et al., 2009 | ||
| Software, algorithm | ComBat | Johnson et al., 2007 | ||
| Software, algorithm | limma | Ritchie et al., 2015 | ||
| Software, algorithm | GSEA | Subramanian et al., 2005 | ||
| Software, algorithm |
Seurat | Butler et al., 2018 | ||
| Software, algorithm | Tableau Public | Tableau Software |