This is measured by fluorescence microscopy in strain AV47 (LC69 HK022::P
127-
msfgfp, λ::P
127-
mcherry)/pCRRNAcos for crRNA or pAV20 for sgRNA. When used to repress the RFP-PBP1a and GFP-PBP1b fusions in AV44, the repression level may be different because of genetic feedback. (
A) Repression of GFP and RFP by crRNAs expressed from the pCRRNAcos vector. (
B) Repression of GFP and RFP by sgRNAs expressed from the pAV20 vector. See
Table 1 for the sequences of the guides. Relative fluorescence is expressed as a percentage of AV47/pAV20 GØ-RØ, that is without repression. Data is from
Vigouroux et al. (2018).