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. 2020 Jan 6;9:e51998. doi: 10.7554/eLife.51998

Key resources table.

Reagent type
(species) or
resource
Designation Source or
reference
Identifiers Additional
information
Strain, strain background (E. coli) LC69 Cui et al., 2018 186::Ptet-dcas9
Strain, strain background (E. coli) AV03 Vigouroux et al., 2018 186::Ptet-dcas9, HK022::P, λ::P
Strain, strain background (E. coli) AV04 Vigouroux et al., 2018 186::Ptet-dcas9, λ::P-mcherry
Strain, strain background (E. coli) AV08 Vigouroux et al., 2018 186::Ptet-dcas9, mrdA::mcherry-mrdA
Strain, strain background (E. coli) AV29 This work 186::Ptet-dcas9, ΔmrcB Supplementary file 1
Strain, strain background (E. coli) AV31 This work 186::Ptet-dcas9, mrcB::msfgfp-mrcB Supplementary file 1
Strain, strain background (E. coli) AV44 This work 186::Ptet-dcas9, mrcB::msfgfp-mrcB, mrcA::mcherry-mrcA Supplementary file 1
Strain, strain background (E. coli) AV47 This work 186::Ptet-dcas9, HK022::P-msfgfp, λ::P-mcherry Supplementary file 1
Strain, strain background (E. coli) AV50 This work 186::Ptet-dcas9, mrcA::mcherry-mrcA, ΔmrcB Supplementary file 1
Strain, strain background (E. coli) AV51 This work 186::Ptet-dcas9, mrcB::msfgfp-mrcB, ΔmrcA Supplementary file 1
Strain, strain background (E. coli) AV58 This work 186::Ptet-dcas9, mrcB::msfgfp-mrcB, ΔmrcA, HK022::Para-msfgfp-mrcB Supplementary file 1
Strain, strain background (E. coli) AV63 This work 186::Ptet-dcas9, mrcA::mcherry-mrcA, ΔmrcB, HK022::Para-mCherry-mrcA Supplementary file 1
Strain, strain background (E. coli) AV67 This work 186::Ptet-dcas9, mrcB::msfgfp-mrcB, mrcA::mcherry-mrcA, ΔpbpC Supplementary file 1
Strain, strain background (E. coli) AV80 This work 186::Ptet-dcas9, mrcB::msfgfp-mrcB, mrcA::mcherry-mrcA, ΔpbpC, ΔmtgA Supplementary file 1
Strain, strain background (E. coli) AV84 This work 186::Ptet-dcas9, mrcB::msfgfp-mrcB, mrcA::mcherry-mrcA, ΔpbpC, ΔmtgA, ΔlysA Supplementary file 1
Strain, strain background (E. coli) AV88 Dion et al., 2019 186::Ptet-dcas9, mreB::mreB-msfGFP
Strain, strain background (E. coli) AV92 This work 186::Ptet-dcas9, mrcB::msfgfp-mrcB, mrcA::mcherry-mrcA, ΔpbpC, ΔmtgA, ΔmscS Supplementary file 1
Strain, strain background (E. coli) AV93 This work 186::Ptet-dcas9, mrcB::msfgfp-mrcB, mrcA::mcherry-mrcA, ΔpbpC, ΔmtgA, ΔmscS, ΔmscL Supplementary file 1
Strain, strain background (E. coli) AV100 This work 186::Ptet-dcas9, ΔmrcA, ΔmrcB, HK022::Para-msfgfp-mrcB Supplementary file 1
Strain, strain background (E. coli) AV101 This work 186::Ptet-dcas9, ΔmrcA, ΔmrcB, HK022::Para-mcherry-mrcA Supplementary file 1
Strain, strain background (E. coli) AV105 This work 186::Ptet-dcas9, ΔmrcB, mrcA::mcherry-mrcA, ΔpbpC, ΔmtgA, ΔlysA Supplementary file 1
Strain, strain background (E. coli) AV109 This work 186::Ptet-dcas9, mrcB::msfgfp-mrcB, mrcA::mcherry-mrcA, ΔlpoA Supplementary file 1
Strain, strain background (E. coli) AV110 This work 186::Ptet-dcas9, mrcB::msfgfp-mrcB, mrcA::mcherry-mrcA, ΔlpoB Supplementary file 1
Strain, strain background (E. coli) AV124 This work 186::Ptet-dCas9, mrcB::msfgfp-mrcB(E313D) Supplementary file 1
Strain, strain background (E. coli) AV128 This work 186::Ptet-dCas9, mrcB::msfgfp-mrcB(E313D), ΔlpoB Supplementary file 1
strain, strain background (E. coli) AV130 This work 186::Ptet-dCas9, mrcB::msfgfp-mrcB(E313D), mrcA::mcherry-mrcA, ΔlpoB Supplementary file 1
Strain, strain background (E. coli) NO34 Ouzounov et al., 2016 mreB::mreB-msfgfpsw-kanR
Strain, strain background (E. coli) B150 This work ΔmrcB Supplementary file 1
Strain, strain background (E. coli) B151 van Teeffelen et al., 2011 FB83, asd-1
Strain, strain background (E. coli) B157 This work FB83, asd-1, ΔmrcB Supplementary file 1
Strain, strain background (E. coli) B172 This work mreB::mreB-msfgfpsw-kanR Supplementary file 1
Strain, strain background (E. coli) B174 This work ΔmrcB, mreB::mreB-msfgfpsw-kanR Supplementary file 1
Strain, strain background (E. coli) B176 This work FB83, asd-1, mreB::mreB-msfgfpsw-kanR Supplementary file 1
Strain, strain background (E. coli) B178 This work FB83, asd-1, ΔmrcB, mreB::mreB-msfgfpsw-kanR Supplementary file 1
Software, algorithm Trackmate Tinevez et al., 2017
Software, algorithm ThunderStorm Ovesný et al., 2014
Software, algorithm SpotOn Hansen et al., 2018
Software, algorithm Morphometrics Ursell et al., 2017
software, algorithm TrackPy Allan et al., 2016
Software, algorithm MicroManager Edelstein et al., 2010