Table A1.
Strains and plasmids used in this study
| Strain/Plasmid | Genotype | Source |
|---|---|---|
| Wild‐type Escherichia coli | BW25113 (F‐, DE(araD‐araB)567, lacZ4787(del)::rrnB‐3, LAM‐, rph‐1, DE(rhaD‐rhaB)568, hsdR514) | Datsenko and Wanner (2000) |
| ecMK94 | BW25113 serA::FRT | This work |
| ecMK101 | BW25113 sdaA::kan | This work |
| ecMK102 | BW25113 sdaCB::cm | This work |
| ecMK210 | BW25113 tdcG::cm | This work |
| ecMK105 | BW25113 serA::FRT sdaA::kan | This work |
| ecMK106 | BW25113 serA::FRT sdaCB::cm | This work |
| ecMK211 | BW25113 serA::FRT tdcG::cm | This work |
| ecMK136 | BW25113/pASEC1 | This work |
| ecMK137 | BW25113/pMKEC24 | This work |
| ecMK147 | BW25113/pMKEC32 | This work |
| ecMK172 | BW25113/pMKEC35 | This work |
| ecMK174 | BW25113/pMKEC36 | This work |
| ecMK176 | BW25113/pMKEC37 | This work |
| ecMK178 | BW25113/pMKEC30 | This work |
| ecMK163 | BW25113 sdaB::kan | This work |
| ecMK164 | BW25113 serA::FRT sdaB::kan | This work |
| ecMK180 | BW25113 sdaC::FRT | This work |
| ecMK181 | BW25113 serA::FRT sdaC::FRT | This work |
| ecMK129 | BW25113 gcvP::kan | This work |
| ecMK196 | BW25113 sdaC::FRT gcvP::cm | This work |
| ecMK184 | BW25113/pMKEC38 | This work |
| ecMK187 | BW25113/pMKEC39 | This work |
| ecMK194 | BW25113/pMKEC41 | This work |
| ecMK221 | BW25113/pMKEC43 | This work |
| ecMK140 | BW25113 serA::FRT/pASEC1 | This work |
| ecMK197 | BW25113 serA::FRT/pMKEC38 | This work |
| ecMK200 | BW25113 serA::FRT/pMKEC41 | This work |
| ecMK142 | BW25113 serA::FRT sdaCB::cm/pASEC1 | This work |
| ecMK198 | BW25113 serA::FRT sdaCB::cm/pMKEC38 | This work |
| ecMK201 | BW25113 serA::FRT sdaCB::cm/pMKEC41 | This work |
| ecMK141 | BW25113 sdaCB::cm/pASEC1 | This work |
| ecMK185 | BW25113 sdaCB::cm/pMKEC38 | This work |
| ecMK188 | BW25113 sdaCB::cm/pMKEC39 | This work |
| ecMK195 | BW25113 sdaCB::cm/pMKEC41 | This work |
| ecMK222 | BW25113 sdaCB::cm/pMKEC43 | This work |
| ecMK186 | BW25113 sdaC::FRT/pMKEC38 | This work |
| ecMK165 | BW25113 alaA::kan | This work |
| ecMK236 | BW25113 sdaB::FRT tdcG::cm | This work |
| ecMK237 | BW25113 sdaB::FRT tdcG::cm sdaA::kan | This work |
| ecMK228 | BW25113 sdaCB::FRT tdcG::cm sdaA::kan | This work |
| MG1655 | F‐, lambda‐, rph‐1 | Blattner et al. (1997) |
| ecMK209 | MG1655 sdaCB::cm | This work |
| pASEC1 | SC101* bla PLtetO1 T7 RBS yfp0 | Subramaniam et al. (2013) |
| pMKEC24 | SC101* bla PserA T7 RBS yfp0 | This work |
| pMKEC30 | SC101* bla PglyA T7 RBS yfp0 | This work |
| pMKEC32 | SC101* bla PsdaCB T7 RBS yfp0 | This work |
| pMKEC35 | SC101* bla Pkbl T7 RBS yfp0 | This work |
| pMKEC36 | SC101* bla PltaE T7 RBS yfp0 | This work |
| pMKEC37 | SC101* bla PgcvTHP T7 RBS yfp0 | This work |
| pMKEC38 | SC101* bla PsdaCB sdaC | This work |
| pMKEC39 | SC101* bla PLtetO1 T7 RBS sdaB | This work |
| pMKEC41 | SC101* bla PsdaCB sdaB | This work |
| pMKEC43 | SC101* bla PsdaCB sdaC E83* | This work |
| pKD13 | oriR6K bla FRT kan FRT | Datsenko and Wanner (2000) |
| pKD32 | oriR6K bla FRT cat FRT | Datsenko and Wanner (2000) |
| pSIM6 | SC101* repAts bla exo bet gam cI857ts | Datta et al. (2006) |
| pCP20 | SC101* repAts cI857ts bla cat FLP | Datsenko and Wanner (2000) |