Fig. 1. ApiAP2^F mutation alters the DNA sequence specificity of the PBANKA_011210 ApiAP2 protein.

(A) DNA sequences that received the highest hit in DNA binding microarray analysis of the first domain of ApiAP2^S- and ApiAP2^F-mutated PBANKA_011210 ApiAP2 proteins. (B to D) PbNK65^S or PbNK65^F parasites were used to infect C57BL/6 mice. RNA-seq analysis was carried out from blood samples of infected mice. Principal components analysis (B), genes whose expressions were significantly altered between the two groups (C), and the pie chart representation of multigene families that these genes belong to (D) are shown. Numbers under bar graphs in (C) indicate the gene annotation in (PBANKA_######) format. Gene families for each gene are indicated in parenthesis. Pseudogenes are shown with asterisks. (E and F) Density graphs showing the relative distribution of the middle hexamer regions of DNA sequences in (A) in the 1500-bp promoter region of randomly sampled (E) or differentially regulated (F) genes.