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. 2020 Feb 5;6(6):eaaw6957. doi: 10.1126/sciadv.aaw6957

Fig. 5. PbNK65F infection leads to a stronger TH1 type immune response.

Fig. 5

(A to G) C57BL/6 mice were infected with either PbNK65F or PbNK65S as described in Fig. 2. Sera were collected at days 7 and 19 after infection and were assayed for total IgM and IgG (A) using ELISA kits. Parasite-specific IgM (B), IgG (C), IgG1 (D), IgG2b (E), IgG2c (F), and IgG3 `(G) were quantified on ELISA plates coated with iRBC lysates of WT parasite– or mutant parasite–infected mice. Arbitrary units (AU) were calculated using a titration curve generated by serially diluting a mixture of serum samples as explained in Materials and Methods. n.s. = P > 0.05; * = 0.01 < P ≤ 0.05; ** = 0.001 < P ≤ 0.01; *** = 0.0001 < P ≤ 0.001 (Welch’s t test).