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. 2020 Feb 5;6(6):eaay0264. doi: 10.1126/sciadv.aay0264

Fig. 3. LINC01356 and LINC00458 are associated with soft matrix–induced and cell type–specific expression signatures.

Fig. 3

(A) Schematic plan to compare lncRNAs between soft and hard substrates. (B) Top 20 differentially regulated lncRNAs in hESCs cultured on soft or hard substrates. (C) LINC01356 and LINC00458 expressions in different human cells by qRT-PCR. hESC, human embryonic stem cell; hiPSC, human induced pluripotent stem cell; THP-1; monocytic leukemia cell; hADSC, human adipose–derived stem cell. The results are presented as means ± SD of triplicates. One-way ANOVA (n = 3 independent experiments). Different letters indicate significant differences, and the same letters indicate no significant difference. (D) Expression levels of LINC01356 and LINC00458 were determined by qRT-PCR after 3 days of culture on the indicated substrate stiffness. The results are presented as means ± SD of triplicates. One-way ANOVA (n = 3 independent experiments). Different letters indicate significant differences, and the same letters indicate no significant difference. (E) Induction of LINC01356 and LINC00458 expressions over 3 days. Cells were cultured on soft and hard substrates. RNA was isolated at the indicated time points and assessed by qRT-PCR. The results are presented as means ± SD of triplicates. ***P < 0.001, **P < 0.005, *P < 0.05, two-sided Student’s t test. (F) Total RNA was isolated from nuclear and cytoplasmic fractions and analyzed by qRT-PCR. MALAT1, metastasis-associated lung adenocarcinoma transcript 1.