Table 1.
qPCR primer sequences
| Primer name | Sequence |
|---|---|
| gpia forward | TCC AAG GAA ACA AGC CAA GC |
| gpia reverse | TTC CAC ATC ACA CCC TGC AC |
| ascl1a forward | GGG CTC ATA CGA CCC TCT GA |
| ascl1a reverse | TCC CAA GCG AGT GCT GAT ATT T |
| stat3 forward | GAG GAG GCG TTT GGC AAA |
| stat3 reverse | TGT GTC AGG GAA CTC AGT GTC TG |
| lin28 forward | CGT GCG GAT GGG CTT CGG ATT TC |
| lin28 reverse | GGC CCC GTC ACT TGT AAG GAC TC |
| ccnd1 forward | CTT ACA CAG AGA AGT TGT G |
| ccnd1 reverse | GGC AAG GAA GTG TTC AAT G |
| ccne1 forward | ACC GGA GAC CTC TGA CTG CT |
| ccne1 reverse | AGC AGG CAG CTC AGC CCT TA |
| ccna2 forward | CGC TAA ACA GGG GTC TGG GC |
| ccna2 reverse | GGT GCT TTC TTG GAG CAC GC |
| cdk4 forward | GCG TTC GGG TGC AGA CCA AT |
| cdk4 reverse | TGA TCC GTC CTG AGG GTC GC |
| cdk6 forward | TCT CAC CGT GTG GTT CAT CGG |
| cdk6 reverse | ATG TCA CAA CCA CCA CGG AAG T |
| id2a forward | CAG ATC GCG CTC GAC TCC AA |
| id2a reverse | CAG GGG TGT TCT GGA TGT CCC |
| p130 forward | AGT CGA GTA ACC GAG CCT GGA |
| p130 reverse | TGG GCA CTG ATG AGC GAC AC |