Figure 6.

Gadd45γ regulates the phosphorylation of downstream transcription factors in hippocampal mouse neuronal cultures. A, Schematic representation of the experimental design. B, Representative immunoblot scans using phosphospecific and total protein antibodies in hippocampal cultures infected with rAAV expressing control–shRNA, Gadd45β–shRNA, Gadd45γ–shRNA1, or Gadd45γ–shRNA2. Hippocampal cultures were harvested at baseline conditions or after 1 h of bicuculline treatment. C–E, Immunoblot quantification of ATF-2 (n = 4–6 independent neuronal cultures; C), c-Jun (n = 5–6 independent neuronal cultures; D), and CREB (n = 5–7 independent neuronal cultures; E) presented in ratios of phosphorylated/total protein normalized to uninfected 1 h bicuculline condition (dashed lines). *p < 0.05, **p < 0.01, ***p < 0.001 by two-tailed unpaired Student's t test. ns, Not significant. Error bars represent SEM.