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. 2020 Feb 5;40(6):1197–1210. doi: 10.1523/JNEUROSCI.1621-19.2019

Figure 8.

Figure 8.

Gadd45γ regulates CREB- and AP-1-dependent transcription in mouse hippocampal cultures. A–E, Schematic representation of the experimental design for qRT-PCR analysis (A) of the expression of the CREB-dependent genes Arc (n = 7–8 independent neuronal cultures; B) and c-Fos (n = 6–8 independent neuronal cultures; C) or the AP-1-dependent genes Nptx2/Narp (n = 6–7 independent neuronal cultures; D) and Tgfb2 (n = 6–7 independent neuronal cultures; E). Hippocampal cultures were harvested at baseline conditions or after 2, 4, or 6 h of bicuculline treatment. Uninfected controls treated with bicuculline were used for normalization at the corresponding peak of gene expression: 2 h (Arc and c-Fos) or 6 h (Nptx2/Narp and Tgfb2; dashed lines). *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001 by one-way ANOVA test followed by Sidak's or Dunnett's post hoc test with pairwise comparisons; #p < 0.05, ##p < 0.01, and ###p < 0.001, Kruskal–Wallis test followed by Dunn's post hoc test for non-Gaussian distribution. ns, Not significant. Error bars represent SEM.