Figure 9.

Gadd45γ reduction does not alter calcium dynamics in response to neuronal activity. A, Schematic representation of the viral constructs used. The viral vector driving jRGECO1a expression does so under control of the human synapsin (hSyn) promoter. Neuronal cultures were either singly infected with jRGECO1a or coinfected with jRGECO1a and control–shRNA, Gadd45β–shRNA, Gadd45γ–shRNA1, or Gadd45γ–shRNA2. bGH polyA, Bovine growth hormone polyadenylation signal; ITR, inverted terminal repeat; WPRE, woodchuck hepatitis virus post-transcriptional regulatory element. B, Schematic representation of the experimental design. C, Images of representative cultured hippocampal cells infected with jRGECO1a at baseline or after ionomycin treatment. Scale bar, 40 μm. D, Representative traces showing the first gabazine-evoked burst of all groups. E, F, Amplitude (n = 6 independent neuronal cultures; E) and frequency (n = 6 independent neuronal cultures; F) of evoked action potential bursts during gabazine treatment in all groups. One-way ANOVA test followed by Sidak's multiple-comparisons test with pairwise comparisons was used. ns, Not significant. Error bars represent SEM.