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. 2020 Feb 5;40(6):1248–1264. doi: 10.1523/JNEUROSCI.1654-19.2019

Figure 1.

Figure 1.

Schematics illustrate the experimental procedures. A–C, Donor cells are dissected from the RN in the GFP transgenic rat embryonic brainstem. Immunostaining for 5-HT indicates (A) the distribution of numerous early-stage serotonergic neurons in the rostral (B) and caudal (C) RN in an E14 brainstem. Dotted lined areas are approximately the regions dissected for cell grafting. D, A diagram represents cell transplantation and neuronal tract tracing. Immediately after spinal cord transection at T4 level (T4-Tx), E14 NSCs are implanted into the lesion site of the spinal cord. Animals survive for 10 weeks. Three weeks before death, BDA is injected into the RVLM of brainstem to anterogradely trace descending vasomotor pathways that regenerated into the graft. FG is injected intraperitoneally to retrogradely label SPNs in the spinal cord. RN-NSC-grafted rats received injections of PRV-614 into bilateral kidneys to retrogradely and transsysnaptically label neurons in the graft and host brainstem. E, A timeline shows the experimental arrangement. Post-g. N, Sympathetic postganglionic neurons. Scale bars: A, 2 mm; C, 200 μm.