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. Author manuscript; available in PMC: 2021 Feb 3.
Published in final edited form as: Analyst. 2020 Feb 3;145(3):816–827. doi: 10.1039/c9an02111f

Table 2:

Observed sequence coverage of E. coli tRNAs by the detected digestion products of RNases T1, cusativin and MC1.

tRNA length (nt) % Sequence Coverage
RNase T1 cusativin MC1 Any two enzymes# Total*
Ala([cmo5U]GC) 76 58 58 61 78 91
Ala(GGC) 76 51 61 72 87 93
Arg([I]CG) 77 69 73 58 66 95
Arg|CCG 77 39 74 39 56 97
Arg([mnm5U]CU) 77 49 53 48 52 91
Asn([Q]UU) 76 61 63 38 42 82
Asp([gluQ]UC) 77 44 66 45 48 84
Cys(GCA) 74 59 69 72 81 100
Gln([cmnm5s2U]UG) 75 77 80 48 81 100
Gln(CUG) 75 76 71 52 75 100
Glu([mnm5s2U]UC) 76 72 75 36 63 96
Glu([mnm5U]UC) 76 72 75 49 67 97
Gly(CCC) 74 64 58 30 43 85
Gly([mnm5U]CC) 75 65 67 59 76 100
Gly(GCC) 76 63 79 55 67 97
His([Q]UG) 77 75 78 43 74 100
Ile|GAU 77 57 58 62 70 90
Ile([k2C]AU) 76 74 63 25 66 91
Leu(CAG) 87 57 64 53 68 95
Leu(GAG) 87 67 53 43 48 89
Leu([cmnm5Um]AA) 87 56 49 47 51 91
Leu([Cm]AA) 85 54 79 65 80 93
Leu([cmo5U]AG) 85 55 60 58 59 98
Lys([mnm5s2U]UU) 76 51 88 61 64 100
Met([ac4C]AU) 77 69 62 65 73 91
Phe(GAA) 76 61 68 42 72 88
Pro(CGG) 77 53 62 34 45 90
Sec(UCA) 89 46 56 39 45 82
Ser([cmo5U]GA) 88 58 60 39 70 89
Ser(CGA) 88 53 50 25 45 75
Ser(GCU) 93 52 58 20 46 87
Ser(GGA) 88 47 50 72 52 99
Thr1(GGU) 76 63 55 43 47 91
Thr3(GGU) 76 66 50 39 54 88
Thr([cmo5U]GU) 76 62 62 47 58 96
Trp(CCA) 76 70 55 28 40 93
Tyr(QUA) 85 74 52 33 49 89
Tyr(QUA) 85 73 56 27 47 92
Val([cmo5U]AC) 76 68 57 54 70 89
Val(GAC) 77 66 52 52 69 92
Val(GAC) 77 57 42 51 48 92
Ini(CAU) 77 56 64 74 61 96
Ini(CAU) 77 56 56 78 62 100
#

refers to the detection and sequence coverage reported by at least two of the three enzymes

*

refers to the total coverage by all three enzymes where detection was made by at least one enzyme.