Figure 4:

Differential expression in nurse shark gill section between the classical UAA and UDA lineages, using in situ hybridization of full-length UAA and UDA region nurse shark riboprobe. A) H&E stained tissue section with the gill filament structures highlighted in the remainder images; B), C) and D) UAA antisense riboprobe; E) and F) UAA sense riboprobe; G), H) and I) UDA antisense riboprobe; J) and K) UDA sense riboprobe; B) E) G) and J) DAPI contain staining; C) H) F) and J) riboprobe with SA-AF647 signal detection and D) and I) riboprobe with signal detection into SA-AP and NBT/BCIP substrate. Note the highest expression of UAA in epithelial cells (white arrows), and of UDA in some pillar (blue arrows) and epithelial cells (white arrows). All images were taken at 20X magnification except the H&E that was taken at 10X magnification.