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Summary of library preparation reagent amounts and incubation times
| Step | Reaction Mix Volume (μl) | Incubation Time (min) | Incubation Temperature (°C) | SPRI Bead Amount (μl) |
|---|---|---|---|---|
| Repair I | 1 (Mix) and 2 (DNA) | 10 | 37 | 4.2 |
| Repair II | 2.5 | 20 | 25 | 3.0 |
| Ligation I | 2.4 | 15 | 25 | 2.0 |
| Ligation II | 2.5 | 10 | 40 | 2.1 |
