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Biosensor peptides can be conjugated onto siRNA-containing LbL liposomes via copper(I)-catalyzed click chemistry to create theranostic LbL NPs. In the tumor microenvironment, biosensor peptides are cleaved, and the resulting fragments can be non-invasively detected in the urine to monitor disease progression. PLR = poly-l-arginine, pPLD = propargyl-modified poly-l-aspartic acid..
