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. Author manuscript; available in PMC: 2020 Aug 1.
Published in final edited form as: Clin Cancer Res. 2019 Oct 21;26(3):690–703. doi: 10.1158/1078-0432.CCR-19-1486

Figure 3.

Figure 3.

CSF1R knockdown impairs T-cell lymphoma growth and survival. A. The efficiency of CSF1R knock-down upon induction (10ng/ml doxycycline) of two independent CSF1R-specific shRNA sequences (shRNA#1 and shRNA #2) or scramble control, as measured by western blot analysis or flow cytometry testing (B). C. Proliferation of T-cell lymphoma lines (Karpas 299) was evaluated after 72hrs of CSF1R shRNA or scramble shRNA induction. D. Representative pictures of colony formation assays in methocult media (1 week growth) upon induction (+dox) or no induction (-dox) of CSF1R-specific or scramble shRNA. E. Bar-graph represents fold-change in the number of colonies when CSF1R-specific or scramble shRNA was induced (Dox) or non-induced (No Dox). Figures are representative of at least three independent experiments. (A) GAPDH as loading control. (C and E) Bars = mean ± SEM. **p < 0.01, n = 5.