Fig. 3.

OMP-52M51 inhibits DLL4-induced Notch activation in CLL. Primary cells from NOTCH1-mutated and NOTCH1-unmutated CLL cases were pretreated for 2 h with OMP-52M51 before DLL4 stimulation (4 μg/mL) for 24 h. a Cleaved Notch1 was assessed by Western blot and densitometrically quantified. β-Actin was probed as a loading control. Representative cases are shown (CLL 2, 8, and 18). b Gene expression analysis by quantitative real time PCR of HES1 and DTX1. mRNA relative levels are given as arbitrary units, using untreated condition as a reference. Control: isotype control. Bars represent the mean ± SEM of NOTCH1-mutated (n = 5) and -unmutated (n = 5) CLL cases. p < 0.05