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. 2019 Oct 15;39(6):1185–1197. doi: 10.1038/s41388-019-1053-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — OMP-52M51 inhibits DLL4-induced CXCR4 expression and migration. Primary cells from NOTCH1-mutated and NOTCH1-unmutated CLL cases were pretreated for 2 h with OMP-52M51 before DLL4 stimulation (4 μg/mL) for 48 h. a CXCR4 expression was analyzed by quantitative real time PCR. mRNA relative levels are given as arbitrary units, using untreated cells as a reference. b CXCR4 expression was analyzed by flow cytometry (n = 4 NOTCH1-mutated cells; n = 4 NOTCH1-unmutated cells). CXCR4 expression levels are showed based on the median fluorescence intensity (MFI) on viable cell population, using untreated cells as a reference. c Samples were assayed for chemotaxis toward CXCL12 (200 ng/ml). Migration is represented as the percentage of migrating cells out of total viable cells added to the transwell. Control: isotype control. Bars represent the mean ± SEM of NOTCH1-mutated (n = 8) and -unmutated (n = 4) CLL cases. *p < 0.05; **p < 0.01. d Samples were assayed for chemotaxis toward CXCL13 (500 ng/ml). Migration is represented as the percentage of migrating cells out of total viable cells added to the transwell. Bars represent the mean ± SEM of NOTCH1-mutated CLL cases (n = 6). *p < 0.05