Fig. 6.

OMP-52M51 inhibits DLL4-induced angiogenesis. Primary cells from NOTCH1-mutated (n = 6) and NOTCH1-unmutated (n = 4) CLL cases were pretreated for 2 h with OMP-52M51 before DLL4 stimulation (4 μg/mL) for 72 h. a Supernatant from CLL cells was harvested after treatment and added to HUVEC cells for 24 h. The number of branch points was quantified as the mean of five randomly chosen fields from each well. Bars represent the mean ± SEM. *p < 0.05. Microscope images (×40 magnification) from a representative case per condition are shown (CLL 2 and 12). b NRARP and VEGFA expression was analyzed by quantitative real time PCR. mRNA relative levels are given as arbitrary units, using untreated cells as a reference. Control: isotype control