TABLE 2.
Summary of the phenotypic switching characteristics and complementation results for the 27 clinical a/α isolates and deletion derivatives of SC5314 and P37039a
| EFG1 genotype | Isolate no. | Strain | Phenotypic transitionb
|
Complementation by scEFG1c
|
||||
|---|---|---|---|---|---|---|---|---|
| Wh to Op | Wh to Gr | Hy | Wh to Op | Wh to Gr | Hy | |||
| EFG1/EFG1 | 1 | SC5314 | − | − | + | |||
| 2 | P37039 | − | − | + | ||||
| 3 | P76035 | − | − | + | ||||
| 4 | P78038 | − | − | − | nt | |||
| 5 | P48086 | − | − | + | ||||
| 6 | P76065 | + | − | + | No | |||
| 7 | P22078 | − | − | + | ||||
| 8 | P75071 | − | − | + | ||||
| 9 | P80004 | − | − | + | ||||
| 10 | P57096 | + | − | + | No | |||
| 11 | P75002 | − | − | − | nt | |||
| 12 | P75058 | − | − | − | nt | |||
| 13 | P80021wh | − | − | + | ||||
| 14 | 529L | − | − | − | nt | |||
| 15 | 1298wh | + | − | + | No | |||
| 16 | P37009 | + | − | + | No | |||
| 17 | P97100 | − | − | + | ||||
| EFG1/efg1Δ | SC5314 | − | − | + | ||||
| P37039 | − | − | + | |||||
| EFG1/efg1− | 18 | P37037wh | + | − | + | No | ||
| 19 | P75063wh | − | − | + | ||||
| efg1Δ/efg1Δ | SC5314 | + | + | − | Yes | Yes | Yes | |
| P37039 | + | + | − | Yes | Yes | Yes | ||
| efg1−/efg1− | 20 | P57003 | + | + | − | Yes | Yes | Yes |
| 21 | P37037dk | + | + | − | Yes | Yes | Yes | |
| 22 | P75063dk | + | + | − | Yes | Yes | Yes | |
| 23 | P52084 | + | + | − | Yes | Yes | Yes | |
| 24 | P75038 | + | + | − | Yes | Yes | Yes | |
| 25 | P75006 | + | + | − | Yes | Yes | Yes | |
| 26 | P80021dk | + | − | − | No | na | No | |
| 27 | 1298dk | + | + | − | Yes | Yes | Yes | |
Summary of the phenotypic switching characteristics, including gray and hypha formation, and complementation results for the collection of 27 clinical a/α isolates and the deletion derivatives of strains SC5314 and P37039. Wh, white; Op, opaque; Gr, gray; Hy, hyphae.
The white-to-opaque transition (Wh to Op) and the white-to-gray transition (Wh to Gr) was tested on GlcNAc-agar under four sets of environmental conditions (data presented in Fig. 2). The yeast-to-hypha formation transition was tested in suspension and on agar plates, both in the presence of 10% serum.
Complementation by scEFG1 represents reestablishment of repression of the white-to-opaque transition, reestablishment of repression of the white-to-gray transition and reestablishment of hypha induction by serum. nt, not tested; na, not applicable; yes, complemented; no, not complemented.