TABLE 3.
Results of complementation experimentsa
| Category | Strain | Switchingb
|
|||
|---|---|---|---|---|---|
| 25°C |
37°C |
||||
| Air | 5% CO2 | Air | 5% CO2 | ||
| Controls | SC5314 efg1Δ/efg1Δ | (+) | (++++) | (++++) | ++++ |
| SC5314 efg1Δ/scEFG1 | − | − | − | − | |
| P37039 efg1Δ/efg1Δ | − | (++++) | (++++) | ++++ | |
| P37039 efg1Δ/scEFG1 | − | − | − | − | |
| EFG1/EFG1 | P76065 EFG1/EFG1 | − | ++++ | ++ | ++++ |
| P76065 EFG1/scEFG1 | − | ++++ | ++ | +++ | |
| P57096 EFG1/EFG1 | +++ | ++++ | − | − | |
| P57096 EFG1/scEFG1 | ++++ | ++++ | − | − | |
| 1298wh EFG1/EFG1 | − | ++++ | − | − | |
| 1298wh EFG1/scEFG1 | − | ++++ | − | − | |
| P37009 EFG1/EFG1 | − | ++ | − | − | |
| P37009 EFG1/ scEFG1 | − | ++ | − | − | |
| EFG1/efg1− | P37037wh EFG1/efg1− | − | − | − | +++ |
| P37037wh EFG1/scEFG1 | − | − | − | ++++ | |
| efg1−/efg1− | P57003 efg1−/efg1− | − | (++++) | (++++) | ++++ |
| P57003 efg1−/scEFG1 | − | − | − | − | |
| P37037dk efg1−/efg1− | (+) | (++++) | (++++) | ++++ | |
| P37037dk efg1−/scEFG1 | − | − | − | ++++ | |
| P75063dk efg1−/efg1− | − | (++++) | (++++) | ++++ | |
| P75063dk efg1−/scEFG1 | − | − | − | − | |
| P52084 efg1−/efg1− | − | (++++) | (++++) | ++++ | |
| P52084 efg1−/scEFG1 | − | − | − | − | |
| P75038 efg1−/efg1− | − | (++++) | (++++) | ++++ | |
| P75038 efg1−/scEFG1 | − | − | − | − | |
| P75006 efg1−/efg1− | − | (++++) | (++++) | ++++ | |
| P75006 efg1−/scEFG1 | − | − | − | − | |
| P80021dk efg1−/efg1− | ++++ | ++++ | − | − | |
| P80021dk efg1−/scEFG1 | ++++ | ++++ | − | − | |
| 1298dk efg1−/efg1− | (++) | (++++) | (++++) | ++++ | |
| 1298dk efg1−/scEFG1 | − | − | − | − | |
Results of complementation experiments, in which one copy of SC5314 EFG1 (scEFG1) was inserted into the native locus at one allele, under the control of native promoter, by site-specific integration in strains that switched to opaque. See Tables S3 and S4 for switching frequencies. Switching analyses were performed on GlcNAc-agar.
++++, 80 to 100% opaque colonies; +++, 21 to 80% opaque colonies; ++, 11 to 20% opaque colonies; −, no opaque or mixed colonies. Parentheses indicates colonies with mixed cellular phenotypes. At 25°C in air or 5% CO2, the mixtures include a majority of opaque cells and a minority of white and gray cells; at 37°C in air, the mixtures include a majority of opaque cells and a minority of white cells (no gray cells). (++++), 80 to 100%; (++), 11 to 20%; (+), 1 to 10%. Each assessment represents the results of pooled data for three independent experiments. The total number of colonies ranged between 425 and 2,059.