FIG 1.
HSV-1 lacking gC exhibits reduced entry and infectivity in a subset of cell types. Equivalent inocula of HSV-1 gCR (A) or ΔgC (B) were bound to Vero, CHO-HVEM, IMR-32, or HEKa cells for 1 h at 40C. Following a shift to 37°C for 6 h, infected cells (MOI of ∼0.9) were quantitated by immunofluorescence. Infectivity is reported as percent HSV antigen-positive cells of ∼500 total cells. Data represent means of results from three independent experiments each performed in triplicate, with standard deviations. *, P < 0.05 (Student's t test).