FIG 2.

Efficiency of gC-negative HSV-1 infection of human cells that support pH-neutral or low-pH entry. (A and B) HSV-1 gCR (A) and ΔgC (B) titers were determined on SK-N-SH or HaCaT cells by plaque assay. (C and D) Attempt to restore infectivity of HSV-1 lacking gC by providing gC in the cell. Equivalent inocula of HSV-1 gCR (C) or ΔgC (D) were added to SK-N-SH or HaCaT cells transfected with empty vector or gC or gD plasmids at 37⁰C. At 18 to 24 h p.i., titers were determined by plaque assay. Data represent means of results from three independent experiments performed in at least triplicate with standard deviations. **, P < 0.01; ns, not significant [Student's t test]).