FIG 4.
HSV-1 ΔgC attachment to cells. (A and B) Approximately 106 genome copies of extracellular HSV-1 gCR (A) or ΔgC (B) were added to the indicated prechilled cell monolayers at 4°C for 1 h on ice. Following two PBS washes, cells were trypsinized, and cell-associated HSV-1 was quantitated by qPCR. CHOpgs745 cells lack heparan sulfate receptors for HSV attachment and served as controls. Data represent means of results from three independent experiments performed in quadruplicate with standard errors. *, P < 0.01 (one-way analysis of variance [ANOVA]).