FIG 7.

The pH threshold of reversibility of gB conformational changes. HSV-1 ΔgC or gCR was treated with pH 7.3 or 5.0 and incubated for 10 min. The pHs of the pH 5-treated samples were increased to the indicated levels for an additional 10 min. Samples were directly blotted to nitrocellulose and probed at neutral pH with gB MAb H126 (A), H1781 (B), SS144 (C), or H1817 (D). MAb reactivity was quantitated, with the pH 7.3-treated sample set to 100%. Data shown are representative of results from two independent experiments.