TABLE 1.
Strain and plasmid list and the APEX2 sequence used in the constructs
| Strain, plasmid, or peroxidase | Genotype, description, or sequence | Vector | Reference or source |
|---|---|---|---|
| Strains | |||
| AFC202 | Wild-type BY4742 with ERG11::ERG11-3xHA | 23 | |
| IP-S7 | Wild-type BY4743 with ERG11/ERG11::3xHA | This study | |
| Plasmids | |||
| pBEVY-L | Empty vector | 24 | |
| pIP9 | APEX2 | pBEVY-L | This study |
| pIP10 | GPD1pro::ERG11-V5-APEX2 | pBEVY-L | This study |
| pIP12 | GPD1pro::ERG11-V5 | pBEVY-L | This study |
| Peroxidase | |||
| APEX2 | GCATCTAGAGCAGGAAAGTCTTACCCAACTGTGAGTGCTGATTACCAGGA
CGCCGTTGAGAAGGCGAAGAAGAAGCTCAGAGGCTTCATCGCTGAGAAGA GATGCGCTCCTCTAATGCTCCGTTTGGCATTCCACTCTGCTGGAACCTTT GACAAGGGCACGAAGACCGGTGGACCCTTCGGAACCATCAAGCAC |