The microbiota structure changes over time and is dependent on the plant fraction. (a) Principal-coordinate analysis of prokaryotic microbiota structure considered by time (samples taken over 5 weeks [weeks 1 to 5]) from M. truncatula grown in Bawburgh soil (all fractions; bulk soil, rhizosphere, rhizoplane, root associated, and phyllosphere, but excluding nodules). (b) Prokaryotic microbiota considered by plant fraction (samples taken over 5 weeks [weeks 1 to 5]) from M. truncatula grown in Bawburgh soil. Nodule samples were only taken at week 5. (c) Taxonomic structure of the total plant microbiota (excluding nodules) of M. truncatula grown in Bawburgh soil over 5 weeks, with the week of sampling indicated. (and b) Error bars indicate the variation between individual samples (for a detailed representation, see Fig. S9), n, number of biological replicates.