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. 2020 Feb 4;11(1):e02807-19. doi: 10.1128/mBio.02807-19

FIG 1.

FIG 1

Identification of MMAR_5438 (EspM) as a DNA-binding protein in M. marinum. (A) The whiB6 gene is separated from the esx-1 locus by the MMAR_5438 gene. The biotinylated 1-kb probe (circles) for the DNA pulldown is indicated. The 500-bp probe for the EMSA analysis is indicated in panel C. (B) MS analysis of the DNA pulldown showing the enrichment of the EspM and HupB proteins. The scale represents normalized MS peak area intensity levels. a.u, arbitrary units. (C and D) EMSAs performed with increasing concentrations of the 6×His-EspM protein from M. marinum (EspMMM) (C) or the 6×His-EspM protein from M. tuberculosis (EspMMT) (D). The control probe used as indicated in both panels was 500 bp of the rpoA open reading frame (ORF) (bp 1309999 to 1310499) from M. marinum. (E) Schematic of the 6×His-EspMMM proteins affinity purified from E. coli used in the EMSAs. (F) EMSA performed with the whiB6-espM probe with increasing amounts of EspMMM, EspMNT, and EspMCT from M. marinum.