FIG 5.

General A. fumigatus functional protein association network based on the protein phosphorylation profile during incubation with Congo red (CR 10 min). The whole set of differentially phosphorylated proteins from the wild-type (WT), ΔsakA, ΔmpkC, and ΔsakA ΔmpkC strains during CR stress was combined for the generation of a general protein association network. Each node represents a protein that is differentially phosphorylated in at least one strain under CR stress. Node colors are divided into gray nodes, for proteins which were not differentially phosphorylated, and heatmap-colored nodes (blue-white-red), for proteins which were differentially phosphorylated in each strain in relation to its control (the same strain without any stress condition). Node shapes represent molecular functions and are divided into triangles (phosphatases), squares (kinases), and circles (other functions). Each edge represents a functional protein association retrieved from the STRING server (medium confidence threshold of 0.4 for the interaction score), and node sizes represent the degree of each node (number of edges connected to the node). It is important to note that not all the differentially phosphorylated proteins are present in the network, as many proteins did not present any functional associations within the whole set. Changes in the color patterns of nodes from panels A to D represent changes in the differential phosphorylation of the proteins comprising the network in WT, ΔsakA, ΔmpkC, and ΔsakA ΔmpkC strains, respectively, during CR stress.