FIG 8.
Phosphomutants for RckA serine phosphorylation sites. (A) Molecular modeling for RckA wild type and predicted phosphomutants. (B to F) Growth phenotypes of the wild type and ΔrckA, RckAS481D, and RckAS481A mutants grown for 5 days at 37°C on minimal medium (MM) or MM plus cell wall-damaging or osmotic stress agents (Congo red [CR], calcofluor white [CFW], caspofungin, and sorbitol). (G) Western blot analysis for MpkA phosphorylation in the wild type and ΔrckA, RckAS481D, and RckAS481A mutants grown for 16 h in MM and transferred to MM plus 300 μg/ml of Congo red. Signal intensities were quantified using the Image J software by dividing the intensity of phosphorylated MpkA (MpkA∼P) by MpkA.