FIG 6.

ES401 eliminates ES114 in mixed-strain aggregates. Fluorescence microscopy images of mixed cultures of GFP-tagged ES114 (cyan) and RFP-tagged wild-type or vasA_2 mutant (T6SS2 mutant) ES401 (magenta) incubated in either low-viscosity liquid (A) or high-viscosity liquid (B) for 12 h. An overlay of the images of ES114 and ES401 is shown in the bottom row. Bars = 5 μm. Each experiment was performed twice with two biological replicates and five fields of view; one representative image is shown.