FIG 2.
Human and CEACAM-humanized mouse neutrophils, rather than macrophages or DCs, bind H. pylori in a HopQ-dependent manner. (A) Micrographs showing the specific binding of H. pylori to murine wt or CEACAM-humanized PMNs expressing human CEACAM receptors. The micrograph shows a merge of CEACAMall (red) H. pylori (green) membranes stained by wheat germ agglutinin (WGA; gray) and nuclei with DAPI (blue). For single stainings, see Fig. S6. Binding of human, murine, or CEACAM-humanized PMNs (B), DCs (C), and macrophages (D) to H. pylori P12-GFP or P12ΔhopQ-GFP was determined by flow cytometry. For all assays, infection was for 1 h at an MOI of 10. n ≥ 3 for all experiments. The data were normalized to uninfected cells and GFP expression. Data were analyzed by two-way ANOVA and the Bonferroni post hoc test. *, P < 0.05; **, P < 0.01; ***, P < 0.001.