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. 2020 Jan 30;10:3062. doi: 10.3389/fimmu.2019.03062

Table 2.

List of primer sequences used for quantitative PCR (qPCR).

Gene Forward primer Reverse primer
HPRT1 5′–TATGGACAGGACTGAACGGC−3′ 5′–ATCCAGCAGGTCAGCAAAGA−3′
MYD88 5′–GCAGCATCCCTTGGATGTCA−3′ 5′–ATCCGACGGCACCTCTTTTC−3′
LBP 5′–CCCAAGGTCAATGATAAGTTGG−3′ 5′–ATCTGGAGAACAGGGTCGTG−3′
IL8 5′–CTGTGAGGCTGCAGTTCTGG−3′ 5′—CCAGGCAGACCTCTTTTCCAT−3′
C3 5′–ATCAAATCAGGCTCCGATGA−3′ 5′–GGGCTTCTCTGCATTTGATG−3′
MPO 5′–CCCGAGTTGCTTTCCTCACT−3′ 5'—AAGAAGGGGATGCAGTCACG−3′
TLR4 5′–TGGTGTCCCAGCACTTCATA−3′ 5′–CAACTTCTGCAGGACGATGA−3'
HIF1A 5′–TGTGTTATCTGTCGCTTTGAGTC−3′ 5′–TTTCGCTTTCTCTGAGCATTC−3′
OCLN 5′–GACGAGCTGGAGGAAGACTG−3′ 5′–GTACTCCTGCAGGCCACTGT−3′
CASP3 5′–ATTGAGACGGACAGTGGGAC−3′ 5′–GCTGCACAAAGTGACTGGAT−3′
VEGF 5′–ATGCGGATCAAACCTCACCA−3′ 5′–TTTCGCTTTCTCTGAGCATTC−3′
OLFM4 5′–CGAATCCCAGTCGGTTTCCA−3′ 5′–TGATTTCCAAGCGCTCCACT−3′