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. 2020 Jan 31;15:717–728. doi: 10.2147/IJN.S232264

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© 2020 Jenab et al.

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MTT assay of PC12 cells was performed to evaluate the toxicity of PAN-kefiran scaffolds after 1, 2, 4, and 6 days. Results confirmed that the PAN–kefiran scaffolds %5 and %10 do not have any toxicity effect on the PC12 cells. Treatment with PAN-kefiran 5% shows significant increase in cell number (compared to control) in 4 days and 6 days after seeding (p≤0.05), but treatment with PAN-kefiran 10% shows no significant increase in cell number compared to control. This increase is indicated by (*). The significant scale is that *p≤0.05, **p≤0.01, ****p≤0.0001.The two-way ANOVA test was used to determine the significant difference between the two means.