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. 2020 Jan 30;10:3111. doi: 10.3389/fmicb.2019.03111

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Copyright © 2020 Xia, Liu, Zhao, Sun, Zheng, Atoni, Hu, Zhang, Zhang and Yuan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Survival analysis, viral RNA detection in tissues, and gross pathology findings of EBIV-challenged mice. (A) Survival curve of mice challenged with serial dilutions of EBIV. Adult mice (n = 5 per group, replicated three times) were challenged intraperitoneally with 10⁶ PFU (dark blue), 10⁴ PFU (dark green), and 10² PFU (dark red) of EBIV. (B) The viral RNA copies in blood, brain, kidney, liver, and spleen tissues were detected by quantitative RT-PCR, and the brain tissue presented a significant difference (*P < 0.05) compared to the other tested tissues. (C) In situ picture of organs in EBIV-infected animals with hemorrhaging and mock-infected animals.