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. 2020 Jan 30;11:11. doi: 10.3389/fimmu.2020.00011

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Copyright © 2020 Jiang, Chen, Hu, Qiu and Zhu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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aCALR effectively suppressed the expression of cytokines and CALR in mice with ALI pre-depleted of MNs. ELISA assay for TNF-alpha, IL-6, and IL-1beta in the lung protein lysates (A) and BAL (B) of treated mice with or without pre-depletion of MNs. (C) ELISA analysis for the expression of IL-18 in lung and BAL. (D) Sandwich ELISA analysis for the expression of CALR in BAL. Samples were measured for three times and representative data of three independent experiments was shown. Data was presented as mean ± standard error. *p < 0.05, **p < 0.01 vs. IgG/LPS group, ^#p < 0.05 vs. IgG/L/LPS group, n = 3.