Figure 8.

Lack of STAT6 abolished the immune suppressive effects of aCALR on STAT6-/- BMDMs. (A) The expression of STAT6 in BMDMs-derived from WT and STAT6-/- mice was analyzed by Western blot analysis. Each lane presents the cell lysates from individual mouse. (B) Sandwich ELISA analysis for the expression of CALR, TNF-alpha, and IL-6 in the supernatants of WT and STAT6-/- mice derived BMDMs. The cells were pre-treated with 1 μg/ml aCALR and then stimulated with 500 ng/ml LPS for 24 h. Data was presented as mean ± standard error, *p < 0.05, **p < 0.05 vs. LPS-treated cells, ^#p < 0.05, ^##p < 0.01 vs. the untreated cells, n = 3. (C) Western blot analysis for p-p38 MAPK, p38 MAPK, and NLRP3 in the treated cells. M indicates protein marker, one representative blot of three independent experiments. (D) The expression of p-p38 MAPK and NLRP3 was quantitatively analyzed. The data was presented as the ratio of p-p38/p38, p-p38/GAPDH, and NLRP3/GAPDH. Two-tailed Student t-test. *p < 0.05 vs. the aCALR-untreated cells.