FIGURE 3.

Presence and loss of fasD in PBTS1, PBTS2, and D188 cultures. PCR analysis with ITS amplification to validate DNA quality, equal concentration, and stability. Amplification of fasD shows its presence in the PBTS1 and PBTS2 pre-cultures (albeit in fewer cells than in D188), and its loss in the PBTS isolates after a single additional passage in rich liquid medium. λ PstI, DNA ladder; NT, no template control. The images of the original gels have been cut and rearranged for a more logic order of the samples. The original images have been provided to the reviewers for evaluation.