Figure 5.

WEE improved the impairment of insulin signaling induced by PA treatment in HepG2 cells. HepG2 cells were incubated with normal glucose (5.5 mM) or high glucose (30 mM) plus PA (0.3 mM) in the absence or presence of WEE (200 and 300 μg/ml) for 24 h and followed by stimulated with insulin (100 nM) for 20 min. Then, the total and phosphorylated forms of IRβ, IRS-1, GSK3β, CREB, c-Jun, FoxO1, Akt, p38, JNK, and ERK were detected by Western blotting.