Figure 7.

WEE suppressed the nuclear translocation of FoxO1 in PA and high glucose treated HepG2 cells. HepG2 cells were incubated with normal glucose (5.5 mM) or high glucose (30 mM) plus PA (0.3 mM) in the absence or presence of WEE (200 and 300 μg/mL) for 24 h and followed by being stimulated with insulin for 20 min. The cytoplasmic and nuclear protein levels of FoxO1 were determined by Western blotting. Data presented in bar charts are mean ± SEM values from six independent experiments. Groups are significantly different from the control group at **p < 0.01. The groups are significantly different from the IR group at ^#p < 0.05 and ^##p < 0.01 determined by Dunnett’s multiple comparisons test (A). The nuclear localization of FoxO1 was determined by immunofluorescence staining. The bar in each photograph indicates 33 μm (B).