Figure 8.

WEE reversed PA induced changes of FoxO1 target genes in PA treated HepG2 cells. HepG2 cells were incubated with normal glucose (5.5 mM) or high glucose (30 mM) plus PA (0.3 mM) in the absence or presence of WEE (200 and 300 μg/ml) for 24 h and followed by being stimulated with insulin (100 nM) for 20 min. The mRNA levels of Pdk4 (A), Dhrs9 (B), and Rbp1 (C) were quantified by qRT-PCR. Data presented in bar charts are mean ± SEM values from three independent experiments. Groups are significantly different from the control group at **p < 0.01. The groups are significantly different from the IR group at ^#p < 0.05 and ^##p < 0.01 determined by Dunnett’s multiple comparisons test.