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. 2020 Jan 30;10:1666. doi: 10.3389/fphar.2019.01666

Figure 9.

Figure 9

WEE promoted the translocation of GLUT2 and GLUT4 in PA treated HepG2 cells. HepG2 cells were incubated with normal glucose (5.5 mM) or high glucose (30 mM) and PA (0.3 mM) in the absence or presence of WEE (200 and 300 μg/ml) for 24 h. The expression levels of GLUT2 in the plasma membrane and cytoplasm fraction were determined by Western blotting (A). After 24 h treatment and followed by exposure to insulin (100 nM) for 30 min. The expression levels of GLUT4 in plasma membrane and cytoplasm fraction were determined by Western blotting (B). The bar graphs represent the relative expression of GLUT2 and GLUT4 in the plasma membrane and cytoplasm fraction. Data presented in bar charts are mean ± SEM values from six independent experiments. Groups are significantly different from the control group at *p < 0.05, **p < 0.01. The groups are significantly different from the IR group at #p < 0.05 determined by Dunnett’s multiple comparisons test.