Table 4.

Results of Virulence Factor Database (VFDB) analysis for the presence of known virulence genes based on whole genome sequencing of isolates of Salmonella utilized for inoculation of pigs in animal studies #2 and #3.

			Derby	4,[5],12:i:-		Typhimurium
VFclass	Virulence factors	Total number of related genes	ISU-SAL242-16	ISU-SAL240-15	ISU-SAL245-16	ISU-SAL243-14	ISU-SAL244-16
Capsule	Vi antigen (Tvi/Vex)	10	0	0	0	0	0
Fimbrial adherence determinants	Agf/Csg	7	7	7	7	7	7
	Bcf	7	7	7	7	7	7
	Fim	9	9	9	9	9	9
	Lpf	5	0	5	5	0	5
	Pef	4	0	0	0	4	0
	Peg	4	4	0	0	0	0
	Saf	4	4	4	4	4	4
	Sef	4	0	0	0	0	0
	Sta	7	7	0	0	0	0
	Stb	5	5	5	5	5	5
	Stc	4	0	4	4	4	4
	Std	3	3	3	3	3	3
	Ste	6	6	0	0	0	0
	Stf	5	5	5	5	5	5
	Stg	4	0	0	0	0	0
	Sth	4	4	4	4	4	4
	Sti	4	4	4	4	4	4
	Stj	5	0	5	5	5	5
	Stk	7	0	0	0	0	0
	Tcf	4	0	0	0	0	0
Macrophage inducible genes	Mig	1	1	1	1	1	1
Magnesium uptake	Mgt	2	2	2	2	2	2
Non-fimbrial adherence determinants	MisL	1	1	1	1	1	1
	RatB	1	1	1	1	1	1
	ShdA	1	1	1	1	1	1
	SinH	1	1	1	1	1	1
Regulation	PhoPQ	2	2	2	2	2	2
Secretion system	TTSS (SPI-1 encode)	30	30	30	30	30	30
	TTSS (SPI-2 encode)	28	28	28	28	28	28
	TTSS−1 and−2 translocated effectors	2	1	1	1	1	1
	TTSS-1 translocated effectors	10	10	10	9	9	10
	TTSS-2 translocated effectors	15	15	15	15	14	13
Serum resistance	Rck	1	0	0	0	1	0
Spv locus	Spv	5	0	0	0	5	0
Stress adaptation	SodCI	1	0	1	1	1	1
Toxin	Typhoid toxin	3	0	0	0	0	0

Green shading indicates that all genes were present for that specific virulence factor; yellow indicates most but not all genes were present.