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. 2020 Feb 5;10:1850. doi: 10.1038/s41598-020-58732-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Surgical procedure and platelet-rich fibrin (PRF) preparation. The Modified Widman flap technique is shown in (A–E). An internal bevel incision was created (A), followed by mucoperiosteal flap reflection. (B) Subsequently, an intrasulcular incision (C) and a horizontal incision (D) were performed to remove the pocket epithelium. Scaling and root planning was done. Lastly, an interdental suture was used for flap closure. (E) For PRF preparation, blood was drawn from jugular vein and centrifuged at 1,300 rpm for 8 min. (F) Subsequently, the PRF was separated from the packed red blood cell layer. (G) The liquid in the PRF construct was removed by gentle gauze compression. (H) In the open flap debridement and PRF treatment (OFD + PRF) group, the PRF was placed over the alveolar bone at the cemento-enamel junction level. Black arrow indicates the PRF.