Table 1.

In vitro and in vivo characteristics of CRASPs^a,b.

		CspA	CspZ	OspE paralogs
Synonyms and other designations		CRASP-1 BbCRASP-1 BBA68 FHBP	CRASP-2 BbCRASP-2 BBH06	CRASP-3 BbCRASP-3 BBN38	CRASP-4 BbCRASP-4 ErpC	CRASP-5 BbCRASP-5 ErpI ErpN ErpA BBP38 BBL39
Gene name		cspA	cspZ	erpP	erpC	erpA
Gene location in B. burgdorferi strain B31		lp54	lp28-3	cp32-9	cp32-2	cp32-1 cp32-5 cp32-8
Gene expression in enzootic cycle	Fed larvae	+	+ (low expression)	+ (high expression)	+ (high expression)	+ (high expression)
	Unfed nymphs	+ (high expression)	–	–	–	–
	Fed nymphs	+ (low expression)	+ (low expression)	+	+	+
	Tick biting sites	+	+ (high expression)	+ (high expression)	+ (high expression)	+ (high expression)
	Dissemination	–	+ (high expression)	+ (high expression)	+ (high expression)	+ (high expression)
FH binding	Purified proteins	+	+	+	-	+
	GOFc	+	+	+	–	+
	LOFd	+	+e	NDf	ND	ND
Additional non-FH ligands related to complement inactivation		C7, C9, FHL-1	FHL-1	CFHR1 CFHR2 CFHR5	CFHR1 CFHR2	CFHR1 CFHR2 CFHR5
Serum resistance	GOFc	+	+	–	–	–
	LOFd	+	+e	+g	–	+g
Infection phenotype	Spirochetes transmission by ticks	Mutant showed defects in surviving at fed nymphs and transmission to hosts	ND	ND	ND	ND
	Spirochete acquisition by ticks	–	–	ND	ND	ND
	Intradermal inoculation	–	Mutant showed defects in bloodstream survival and tissue colonizationc	ND	ND	Mutant showed defects in tissue colonizationh

^aTable adapted from Kraiczy and Stevenson (2013).

^bDifferent information may be shown because of different strains used to define that information. The information here is derived from B. burgdorferi strain B31.

^cProduced in a gain-of-function background (GOF).

^dProduced in a loss-of-function background (LOF).

^eOnly in blood treated condition.

^fNot determined.

^gOnly when ErpP and ErpA are expressed under flaB promoter in a cspA-deficient B. burgdorferi in the infectious background.

^hPerformed using a transposon-inserted erpA mutant in an infectious B. burgdorferi background.