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. 2020 Feb 5;11:722. doi: 10.1038/s41467-019-14172-4

Fig. 10. CD47-activating peptide treatment alters mesenchymal stem cell differentiation independent of the action of SIRPα.

Fig. 10

a PBS and p7N3 (CD47 agonist) treatment combined canonical correlation analysis and T-distributed stochastic neighbor embedding (t-SNE) plot identified 16 distinct cell clusters based on gene expression differences. b Violin plots for expression of chondrogenic and osteogenic genes. c Immunofluorescence for SOX9 in p7N3 (CD47 agonist) treated and PBS vehicle control specimens 10 days after burn/tenotomy. Scale bar represents 100 μm. All pairwise comparisons were analyzed for homoscedasticity and difference in means via Levene’s F-test and two-tailed Student’s t-test, respectively. *p < 0.05, **p < 0.01. d Cartoon diagram of macrophage participation in the formation of HO and mechanism of action of p7N3, CD47-activating peptide treatment. e Normalized expression of Nos2, Arg1, Mrc1, and Tgfβ1 from macrophages isolated from the HO site after burn/tenotomy and treatment with vehical (veh) or anti-SIRPα. Source data are provided as a Source Data file.