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. 2020 Feb 5;11:722. doi: 10.1038/s41467-019-14172-4

Fig. 2. Monocyte-derived macrophages are important in the initial immune response to musculoskeletal injury and persist 3 weeks after injury.

Fig. 2

a Immunofluorescence of LysmCre+/−/mTmGfl/fl mice at 1 and 3 weeks after injury localizing EGFP+ myeloid cells. Scale bars correspond to 100 μm. b In vivo measurement of inflammation with imaging of myeloperoxidase (MPO) activity. n = 3 mice per indicated time point. Top: representative images of each time point (scale shown: blue = low, red = high). Bottom: quantification of the total bioluminescent signal at the injury site using standardized region of interest (ROIs). c Gating strategy for flow cytometry analysis to identify inflammatory cell populations. d Left: Quantification of recruitment of inflammatory cell populations over time was analyzed using flow cytometry (n = 4 mice per time point). Right: Representative flow cytometry plots demonstrating dynamic changes in Ly6C, F4/80 and CD206 monocyte populations over 3 weeks (n = 4 mice per time point). Source data are provided as a Source Data file.